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KMID : 0379120130410040248
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Korean Journal of Mycology
2013 Volume.41 No. 4 p.248 ~ p.254
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Isolation of the Bacterium Pseudomonas sp. HC1 Effective in Inactivation of Tolaasin Produced by Pseudomonas tolaasii
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Lee Chan-Jung
Yoo Young-Mi
Han Ju-Yeon
Jhune Chang-Sung
Cheong Jong-Chun
Moon Ji-Won
Suh Jang-Sun
Han Hye-Su
Cha Jae-Soon
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Abstract
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A Gram-negative bacterium was isolated from mushroom media that markedly reduces the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. The HC1 strain was selected as detoxifying tolaasin by bioassay on potato and it was identified Pseudomonas sp. by the cultural, morphological and physiological characteristics, and analysis of the 16S rRNA.. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell, was sufficient for detoxification in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Pleurotus ostreatus, Flammunia velutipes and Agaricus bisporus. Control efficacy of brown blotch of strain HC1 treatment was 69, 68 and 55% on Agaricus bisporus, Flammulina velutipes and Pleurotus ostreatus, respectively. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by P. tolaasii.
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KEYWORD
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Control efficacy, Detoxify, Mushrooms, Pseudomonas tolaasii, Tolaasin
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